Validation of Spectrophotometric Method for Analyses of Anionic Surfactant Dodecyl Benzene Sulfonate (DBS) in Catfish (Clarias batrachus) Using Malachite Green

Hermawan Purba1, Adhitasari Suratman2, and Eko Sugiharto2

1Department of D-III Health Analyst, Sekolah Tinggi Ilmu Kesehatan (STIKes) Senior – Medan; 2Department of Chemistry,  Faculty of Mathematical and Natural Sciences, Gadjah Mada University – Yogyakarta, INDONESIA

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Validation method of DBS anionic surfactant analysis on Clarias batrachus has been conducted. The method of analysis was divided into two phase, namely the extraction  with solid-liquid extraction using Soxhlet and analysis DBS. The extraction was performed using n-hexane and methanol for 9 and 6 hours respectively. The analysis was performed using Spektrophotometer UV-Vis based on the complex formation of surfactant-malachite green (DBS-MG). These methods are applied to determine DBS accumulation of Clarias batrachus with DBS concentration exposure and DBS concentration of Clarias batrachus in markets. The result showed that the parameters of validation methods has high acceptability as linearity (R2 = 0.99), limit of detection (LOD) and limit of quantification (LOQ) (0.029 mg/L and 0.089 mg/L), sensitivity (ε = 38.15 × 104 L mol-1 cm-1), precision (RSD = 0.10-1.83 %) and accuracy (recovery = 80-92 %). The result of DBS analysis in Clarias batrachus with 2.5, 5.0, 10.0, 15.0 mg/L of DBS concentration exposures obtained 5.5, 6.8, 7.9, 8.7 mg/L respectively and Clarias batrachus from markets in a range 2.0-4.2 mg/L. The result showed that the analysis of DBS anionic surfactants using MG can be applied for Clarias batrachus.

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