Characterization of Cinnamadehyde Compound Isolated from Cinnamon Oil and Its Salmonella Typhy Antibacterial Activity

Budiana I Gusti M. Ngurah1, Moses K. Tokan2, and Agus Saputra3

1Chemistry Study Program,  Faculty of Teacher Training and Educational Sciences, Nusa Cendana University ,2Biology Study Program, Faculty of Teacher Training and Educational Sciences, Nusa Cendana University,3Faculty of Veterinary, Nusa Cendana University, INDONESIA

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Today many infectious diseases are common. All of the diseases are caused by agents such as viruses or bacteria which are pathogenic and Salmonella typhi  is one of  the pathogenic bacteria. In addition, many antibiotics are not able to work properly because of the resistance of bacteria against the exciting antibiotics. Therefore, research to discovered new anti-bacterial compounds derived from natural materials is important to do. This research was conducted in several stages, such as; (1) Isolation of Cinnamaldehyde from cinnamon oil by two methods: thin layer chromatography method using n-hexane : ethanol eluent at a ratio of 5 : 95 and bisulfate addition  method. (2) Identification of cinnamaldehyde by UV-Vis Spectrophotometer and GC-MS to define its purity. (3) Antibacterial activity test of cinnamaldehyde to the growth of  Salmonella typhi  with different concentrations of  cinnamaldehyde solution i.e 20 ppm, 40 ppm, 60 ppm, and 100 ppm. (4) Then the growth of the tested bacteria was observed by using a colony counter to see the diameter of the resistance which was caused by the test solution.  Analysis result of Gas Chromatography – Mass Spectrophotometer showed that cinnamon oil (Cassia oil) contains cinnamaldehyde as the main component of 88.33 %. The isolation of cinnamaldehyde by bisulfate addition method obtained cinnamaldehyde as yellow fluid with a yield of 86.79 %.. The result of antibacterial activity test indicated that cinnamaldehyde had the potential as an antibacterial to against the Salmonella typhi seen from its inhibition zone. At the concentration of 100 ppm, cinnamaldehyde solution showed an inhibitory diameter of 15,4 mm and amoxicillin antibiotic showed  of 16,6 mm.

Cited references

Pedraza-Chaverri J, Cárdenas-Rodríguez N, Orozco-Ibarra M, Pérez-Rojas JM. Medicinal properties of mangosteen (Garcinia mangostana). F. 2008;46(10):3227-3239. doi:10.1016/j.fct.2008.07.024
ITO C, MIYAMOTO Y, RAO KS, FURUKAWA H. A Novel Dibenzofuran and Two New Xanthones from Calophyllum panciflorum. C. 1996;44(2):441-443. doi:10.1248/cpb.44.441
Likhitwitayawuid K, Chanmahasathien W, Ruangrungsi N, Krungkrai J. Xanthones with Antimalarial Activity fromGarcinia dulcis. P. 1998;64(03):281-282. doi:10.1055/s-2006-957429
Varache-Lembège M, Moreau S, Larrouture S, Montaudon D, Robert J, Nuhrich A. Synthesis and antiproliferative activity of aryl- and heteroaryl-hydrazones derived from xanthone carbaldehydes. E. 2008;43(6):1336-1343. doi:10.1016/j.ejmech.2007.09.003
Riscoe M, Kelly J, Winter R. Xanthones as Antimalarial Agents: Discovery, Mode of Action, and Optimization. C. 2005;12(21):2539-2549. doi:10.2174/092986705774370709
Shan B, Cai Y-Z, Brooks JD, Corke H. Antibacterial Properties and Major Bioactive Components of Cinnamon Stick (Cinnamomum burmannii):  Activity against Foodborne Pathogenic Bacteria. J. 2007;55(14):5484-5490. doi:10.1021/jf070424d
Budiana I Gusti M. Ngurah BIGM. Synthetic C-Methoxyphenyl Calix [4] Resorcinarene and Its Antioxidant Activity. J. 2018;5(1):403-408. doi:10.22341/jacs.on.00501p403

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