Budiana I Gusti M. Ngurah1, Moses K. Tokan2, and Agus Saputra3
1Chemistry Study Program, Faculty of Teacher Training and Educational Sciences, Nusa Cendana University ,2Biology Study Program, Faculty of Teacher Training and Educational Sciences, Nusa Cendana University,3Faculty of Veterinary, Nusa Cendana University, INDONESIA
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Today many infectious diseases are common. All of the diseases are caused by agents such as viruses or bacteria which are pathogenic and Salmonella typhi is one of the pathogenic bacteria. In addition, many antibiotics are not able to work properly because of the resistance of bacteria against the exciting antibiotics. Therefore, research to discovered new anti-bacterial compounds derived from natural materials is important to do. This research was conducted in several stages, such as; (1) Isolation of Cinnamaldehyde from cinnamon oil by two methods: thin layer chromatography method using n-hexane : ethanol eluent at a ratio of 5 : 95 and bisulfate addition method. (2) Identification of cinnamaldehyde by UV-Vis Spectrophotometer and GC-MS to define its purity. (3) Antibacterial activity test of cinnamaldehyde to the growth of Salmonella typhi with different concentrations of cinnamaldehyde solution i.e 20 ppm, 40 ppm, 60 ppm, and 100 ppm. (4) Then the growth of the tested bacteria was observed by using a colony counter to see the diameter of the resistance which was caused by the test solution. Analysis result of Gas Chromatography – Mass Spectrophotometer showed that cinnamon oil (Cassia oil) contains cinnamaldehyde as the main component of 88.33 %. The isolation of cinnamaldehyde by bisulfate addition method obtained cinnamaldehyde as yellow fluid with a yield of 86.79 %.. The result of antibacterial activity test indicated that cinnamaldehyde had the potential as an antibacterial to against the Salmonella typhi seen from its inhibition zone. At the concentration of 100 ppm, cinnamaldehyde solution showed an inhibitory diameter of 15,4 mm and amoxicillin antibiotic showed of 16,6 mm.